Sentence examples for strict blast from inspiring English sources

Those subfamilies pairs can be joined in one bigger subfamily with less strict Blast parameters.

As described earlier, reads were assigned to reference genomes based on a strict Blast identity cut-off (95%) identity.

Relying on a strict BLAST threshold may exclude homologous proteins whose sequence similarity is not high (false negatives).

The failed samples did not overlap in many cases and this could have been in some cases due to strict BLAST analysis criteria and new splice isoforms seen on the agarose gels.

In the standard approach (Fig. 1), contigs were searched against the refseq_protein database (downloaded from NCBI 14 June 2011) with strict BLAST-settings (BLASTx parameters: threshold 999, window-size 4, gapopen 32767, gapextend 32767, E-value 1e−20) (Altschul et al., 1990).

Special treatment of regions with ambiguous alignment was not necessary, because the orthologous sequences, selected by the strict reciprocal Blast hit method, were on average 61% identical with the standard deviation of ± 18%.

236 families of putatively orthologous genes were detected using the strict reciprocal best Blast hit method [ 46, 47, 51, 52] with an E-value cutoff of 10-4.

Information about these organisms is listed in Table  3.> To construct the gene family phylogenetic tree, the strict reciprocal best BLAST hit method [ 20, 21] with E-value cutoff 10−5, identity >25%%, and alignment length >60 % was used to identify orthologous gene families.

For a set of 13 gamma proteobacetria 236 gene families were assembled using the strict reciprocal top scoring BLAST hit method (see Methods for details).

One miRNA, mir-1949, is previously known only for rodents, and could also be a mis-annotation induced by the otherwise strict filtering of the BLAST results.

To identify the genomic region in G. gallus that contained the claw, feather, and feather-like β-keratins described by Presland et al [ 12] we used very strict E-values and BLAST scores.