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C. butyricum is a strict anaerobe, and maintains a balanced redox by utilizing two pathways (Figure 2).

Only P. intermedia yields a slightly higher value, which may be a result of handling during sample treatment, considering that this pathogen is a strict anaerobe.

The members of bacterial communities during H2 production were mainly identified as facultative anaerobes, belonging to the Enterobacteriaceae and several species of the strict anaerobe Clostridium[1].

F. succinogenes is a strict anaerobe that produces a mixture of succinate, acetate and formate as fermentation end products [3], [7].

A similar dNTP extra supply has been described in the strict anaerobe Bacteroides fragilis, which has both class I and class III enzymes.

P. gingivalis, a gram-negative strict anaerobe and successful colonizer of oral tissues, can survive, replicate, and spread in GECs [18], [36].

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Similar(35)

Additionally, the oxidative phosphorylation gene clusters with sequence similarity to strict anaerobes explain the anaerobic respiratory versatility of A. dehalogenans.

The major example is oxygen that is been used by the aerobes from the upper intestinal tract inducing a deep anaerobic state in the distal intestine allowing hence strict anaerobes to survive.

However, it is possible that some strict anaerobes might be affected by oxygen during this procedure, so anaerobic application of the latex biofilm may be of interest in future studies.

Strict anaerobes such as clostridia have been the most widely studied among the various anaerobic and facultative anaerobic bacteria capable of fermentative hydrogen production [ 4].

Organisms that grow in the absence of free oxygen are termed anaerobes; those that grow only in the absence of oxygen are obligate, or strict, anaerobes.

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