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The probability and frequency of Ca2+ sparks induced by stretch were closely related to the extent of cell extension and the time that the stretch was maintained.
The probability (percent of experiments or cells with Ca2+ sparks) and frequency of Ca2+ release events induced by stretch were highly related to both the degree of stretch and the time that stretch was maintained.
Compared values were the integral of the EMG signal in 5 second time period in which the pre-manipulative stretch was maintained.
The EMG signals were smoothed with a root mean square function with a 50 msec sliding window and the area under the processed signals was integrated within five seconds, during which the pre-manipulative stretch was maintained in order to provide quantitative measures of the amount of muscular activation.
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Although spasticity is velocity-dependent, surface EMG recordings show that in many cases if the stretch is maintained (velocity = 0), the muscle still keeps contracting, at least for a time.
Stretching intensity was maintained at moderate intensity, as recommended by American College of Sports Medicine guidelines.
The temperature of the stretch device was maintained at 37°C.
However, it is important to note that mechanosensing per se was not affected by loss of miRNAs since acute stretch-induced MAPK activation was maintained in Dicer KO vessels.
Basal tone was recorded when it reached a peak after stretching and when re-stabilization (plateau) was maintained for at least 30 minutes, immediately before obtaining concentration-response curves to ACh.
The stretching surface is maintained at a constant temperature and with significant Hall currents.
Extending horizontally was done by delaying between changing the outgoing color value, while vertical stretching was accomplished by maintaining a counter and only unpacking the next line when the counter indicated to.
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