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After a 10 or 60 minute stretch period, wells (N = 12 per group) were removed from the stretch device and mounted into Ussing chambers within approximately 2 minutes.
Following the stretch period (0 or 60 minutes), cells (2 isolations of 2 wells per group) were washed with phosphate-buffered saline (PBS), fixed for 15 min with 1.5% paraformaldehyde, washed again in PBS, and treated for 5 min with 0.1% Triton X-100 in PBS.
Following the 60 minute stretch period, wells were imaged in three locations (20× objective) using a fluorescent microscope (Nikon TE-300, Melville NY), the number of dead and live adherent cells were determined, and toxicity was calculated as the ratio of dead cells divided by the total number of cells (dead plus live cells).
Similar(57)
Usually, when the stretching period is scheduled to end, Fassel must blow his whistle two or three times to interrupt the jocularity surrounding him.
The stretching rate was sufficiently high that observation of low viscosity Newtonian fluid stretching, end pinching and break was observed during the stretching period of the deformation, whereas for a higher Newtonian viscosity, filament thinning and breakup occurred after the cessation of piston movement.
Each exercise session lasted 40 to 45 minutes, in addition to a five-minute stretching period.
During the initial stretching period, the PDMS substrate elongates along the stretching axis and compresses along the perpendicular axis.
so take that into consideration when stretching, period.
With "Fast Five," Universal decided to stretch the period, using the marketing slogan "summer starts early".
Reservoirs created by damming rivers are often believed to increase malaria incidence risk and/or stretch the period of malaria transmission.
The complexity of the case stretched that period out to more than three years.
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