We chose H2O2 because of our experience with this oxidative stressor; experiments were performed as described by us previously [21], [49], [50].
Indeed in multiple stressor experiments investigating the interacting effects of temperature with, for example, lowered pH, the dominant effect has been identified as temperature [ 4– 6].
For each stressor, three independent experiments were performed.
In our model, the symbols P, T, E and TE represent the effects of probe, treatment, experiment per stressor (1, 2, or 3) and the interaction between treatment and experiment, respectively.
In assessing risk to marine biota from climate change it is critical to investigate interactive effects of stressors in multifactorial experiments as this better reflects the real world scenario [1], [7].
In accord with previous research on hostility evaluating cardiovascular deregulation in hostile men using left and right frontal fluency stress [21, 75], the present experiment employed lateralized stressors in this population with a diminished capacity for negative emotional regulation.
Obviously, detection of a response to a stressor is best accomplished by experiments in which the stressor can be manipulated in frequency, intensity, and duration.
These procedures were later modified by Willner resulting in higher length of experiment and lower severity of stressor [ 16].
Hypotheses related to cardiovascular responses to lateralized neuropsychological stressors were generally supported by the current experiment.
In these experiments we used the ER stressor tunicamycin or ionomycin as controls whose apoptotic effects are influenced by the expression level of BiP (23, 24).
