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Functional studies of the interactions among and between mRNAs and μRNAs will provide additional experimental data for validation of the relationships on the level of mRNAs, miRNAs and organismal phenotype that were stressed in this study.
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The calculation of traction stresses in this study was accomplished by generating a finite element mesh surrounding a cell in a hydrogel using confocal images.
Hence, it was not surprising that we found genes related to stress in this study.
Feedback data is displayed in figures, but feedback was not included in ANOVAs because we intended to study responses related to stress in this study.
In general, the HIPVs detected upon biotic and abiotic stresses in this study agree with those associated with biological damaging events [ 9, 13, 22, 43] and with indirect plant defense [ 20, 25, 30, 44].
All these operons, except for CAC2705, were highly overexpressed during the time course of all the tested butanol and butyrate stresses in this study, which is consistent with previous reports.
Collectively, we suggest that the pink salmon were more stressed overall in this study.
It has to be stressed that in this study I3C and DIM in the dose (100 μM) used by Wang et al. [ 30] showed significant cytotoxicity.
Comparison of the transcriptional response to the stresses tested in this study (heat shock, oxygen exposure and osmotic downshift) allowed identification of a set of core stress response genes that is not specific to any single stimulus (Table 4).
For example, miR2871b was induced by all three abiotic stresses examined in this study.
Commonly ten BrLIMs among them, showed differential expressions in response to the abiotic stresses applied in this study.
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