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To avoid clues projected by the stressed animals, all non-stressed animals were tested before stressed animals and the stress room was cleaned thoroughly in between.
Moreover, CCKR-2 agonists could only produce, or produce more pronounced, anxiogenic effect in stressed animals, but not in un-stressed animals [53], and patients with anxiety were more sensitive to CCKR-2 agonists than normal controls [54].
Control non-stressed rats are tested in a breeding cage as stressed animals.
Non-stressed control mice were kept in their familiar environment, isolated from stressed animals and noise.
Those chronically stressed animals were then compared with nonstressed peers.
Quercetin alleviated behavioral dysfunction in chronically stressed animals.
When they gave it to stressed animals, they bounced back faster and acted less depressed.
Safranal ameliorated the changes in the stressed animals as compared with the control groups.
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There was also a trend for thinner fibrous caps in the stressed mice compared with the non-stressed animals (Fig. 4C).
Furthermore, an acute administration of the β-blocker, nadolol, significantly attenuated this effect of stress, without affecting survival rates of non-stressed animals.
Both these drugs when given alone to non-stressed animals did not alter basal levels of antioxidant defense components and oxidative stress markers significantly.
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