He insisted, however, that we must bear "the undoubtedly bad effects" of moral progress, strengthening this point in the second edition of the Descent: We cannot check our sympathy "even at the urging of hard reason, without deterioration in the noblest part of our nature" (Ibid. 134).
The fact that overexpression of miR-302, but not miR-367, is required to fully reprogram somatic cells to a competent pluripotent state 13 indicates functional differences between these two related miRNA, strengthening this point.
To this end, I have strengthened this point in the paper.
The authors should provide CMAC staining in all these strains to strengthen this point, since the vacuole could be fragmented in the vps52Δ.
Regarding the U4-U6 stem-Prp3 interaction, further experimental details should be added, along the lines suggested by Reviewer 3, to strengthen this point.
The report of Grasl-Kraupp et al (1995), which found that TUNEL failed to distinguish between apoptotic and necrotic cells, strengthens this point.
To strengthen this point, we have substantially revised Figure 2, added Figure 2 figure supplement 2, and expanded the text describing the MNase sensitivity (Results section entitled "cenH3 peaks are hyper-sensitive to MNase digestion").
To further strengthen this point a representative curve of one child illustrating, a poor effort performed at teaching process vs. end of test curve is shown in figure 4. The poor-effort curve did not fulfill start of test criteria and is round while the "end of test curve" has an obstructed shape.
Danger of Venetian assault suddenly increased in the times of the First Holy League, and it was necessary to strengthen this vulnerable point of the city fortifications.
To strengthen this particular point we therefore turned back to the constitutive overexpression system, which a) allows for more uniform maintenance of Id1 activity throughout the culture and b) allows for a more rapid 'escape' into differentiation.
However, to strengthen this critical point they should determine blood/tissue partitioning, using in vivo CD45 labeling, for instance as recently reported by Girgis et al. (PLOS Pathogen 2014), at least in their flow cytometric analysis but preferentially also in the imaging.
