Exact(3)
As external knobs to control the state of the cell, the inducer IPTG modulates the strength of repression of LacI, whereas ATc modulates the strength of repression of TetR.
The GAL network allows for a larger phenotypic divergence in the uninduced state (consistent with our observations of larger variance in path scores pertaining to switch effectiveness and strength of repression).
In those rare cases of multiple regulation, the full extent of regulation would not be observed in our transcriptome analysis, but this does not affect our analysis since we are looking at the relative strength of repression at different CcpA concentrations.
Similar(57)
In addition, the strength of the repression, i.e the concentration of the preferred substrate in the medium under which the repression is fully operating, can greatly differ depending of the regulatory circuit of the second substrate usages.
Dual-reporters carrying the partially complementary sequence (mi reporter: mis-matched at position 11 to 13 and the last two nts in miRNA/mRNA duplex) of a miRNA in the 3'UTR of Renilla were used to measure the strength of translational repression from the corresponding miRNA.
Having three perfect sites rather than one would actually underestimate the strength of Luc repression due to miR-100 transfer.
A pure Her7 oscillator with homodimerization of Her7 prior to DNA binding (which determines the strength of the repression) had a period of 30 minutes.
Strength of translation repression (1×-6×) was deduced by comparing the effect of the uAUG either to a control construct or to a construct where the uAUG under consideration is mutated or deleted.
Tet-ON levels will increase depending on the strength of self-repression, activating GFP expression.
The same NF-κB complex thus can simultaneously activate and repress distinct κB sites located in the same promoter where the strength of activation and repression dictates the overall outcome.
The resulting regression coefficients of each miRNA are interpreted as the strength of miRNA-mediated repression of the target mRNA.
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