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The major positive of this process is that the move is an initial step towards a more approachable and streamlined protocol on what to do about sampling.
A potential limitation of the AusHSI streamlined protocol is that the process may not work on a large scale.
This streamlined protocol aims to reduce the content and time required by applicants and reviewers and provide rapid and timely decisions on funding outcomes, whilst still providing transparent review with written feedback to assist improved resubmissions.
The OpenArray® system has an easy work flow and streamlined protocol were most liquid handling steps are automated and enables progression from cDNA into results in less than three hours.
Diffusion tensor imaging (DTI) data at 43 μm isotropic resolution has been acquired on the intact adult mouse brain in 28-h scan time by using a streamlined protocol, including specimen fixation and staining, image acquisition, reconstruction, post-processing, and distribution.
Some applicants, including those who were not funded, provided feedback on the streamlined protocol.
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Novel reagents and streamlined protocols for rapidly assembling co-expression constructs for this purpose have been designed and validated.
We have improved and streamlined protocols to circumvent possible size bias, to simultaneously generate ORF constructs with and without stop codons, and to automate most of the processes.
Subsequent access to these resources enabled the following simplified, more streamlined recombineering protocol to be developed requiring only a single PCR to generate the initial RT-cassette, smaller culture volumes and incubation times, and no formal requirement to sequence final recombinants.
In the process of establishing TALENs as tools for genome modification in Platynereis, we streamlined several protocols together into a single workflow (for a summarizing workflow scheme, see also Zantke et al. 2014).
LV vectors can be produced at much higher titre (in 2012 GMP grade LV vectors were typically supplied in the 10 10 Ig/ml range) and enable streamlined transduction protocols.
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