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The swab was used to streak the agar plate to produce a lawn of growth by streaking the plate in 3 different directions.
Again, each mixture was exposed to the sliding friction field between a streak bar and agar hydrogel for 60 sec.
Each mixture was exposed to sliding friction between a streak bar and agar hydrogel for 60 sec to form P. pseudoalcaligenes penetrons.
The mixture was exposed to the sliding friction between the streak bar and agar hydrogel for 60 sec to form penetrons.
Samples were streaked onto agar plates containing 1.5% agar and the culture medium.
The cultures were streaking on agar plates added with phenol and cyanide and incubated at 30 °C for 48 h.
Both infected and non-infected fragments were crushed and streaked on agar plates to determine mean CFU's, as previously described.
Parental strain MGAS2221 containing vector pDC123 gave a typical β-hemolytic morphology when streaked onto agar plates containing 5% sheep blood.
Upon streaking on agar plates, spheres produced normal colonies, which confirmed the preservation of propagation.
Cells from each isolate were streaked on agar medium containing 1.0 mg/ml FOA at 30°.
His+ transformants were selected and streaked on agar medium containing FOA.
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