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Yet, recent stratum formation or added genes from autosomes have not been reported for the well-studied Y chromosomes of two other carnivores, cat and dog (Cortez et al. 2014).
The absence of segmental structure within chicken autosomal sequences homologous to human X-linked sequences demonstrates that stratum formation fundamentally alters sequence composition, thus allowing identification of compositional signals unique to evolutionary strata by the proposed segmentation and clustering method.
Finally, due to the paucity of information on bear sex chromosomes, we cannot exclude the possibility of recent stratum formation, with the existence of segments that have not yet attained a high level of divergence between the Y and X chromosomes.
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AMEL was clearly a problem for the Lahn and Page (1999) model of strata formation by Y inversion until it was found that AMELX and AMELY showed evidence for gene conversion, which could explain the peculiar divergence pattern of AMEL (Marais and Galtier 2003; Ross et al. 2005).
In PPAR-α /– mice we observed delayed stratum corneum formation between day 18.5 of gestation and birth.
This study was designed to test whether PPAR-α plays a physiologic role in epidermal differentiation and stratum corneum formation in utero.
Furthermore, we have reported that both epidermal differentiation and stratum corneum formation in utero are stimulated by pharmacologic activation of PPAR-α.
Histological analysis by hematoxylin and eosin (H&E) staining (Supplementary Figure S2B) and fluorescent labeling of DNA by Hoechst 33258 (Figure 2D) demonstrated that skin models, in which the expression of serpinB1 was suppressed, were able to completely degrade nuclear DNA during stratum corneum formation.
To test whether serpinB1/L-DNase II contributes to the acid DNase activity of epidermal keratinocytes, we knocked down the expression of serpinB1 with specific siRNAs in human organotypic skin cultures and compared the stratum corneum formation and DNase activities of these cultures with that of cultures treated with control siRNA.
KLK5 and KLK7 are involved in keratinization, stratum corneum formation, turnover and desquamation of the skin, via degradation of the cell adhesion glycoproteins, corneodesmosin and plakoglobin [ 59– 61].
These mice present with degeneration of hair follicles and dermal cysts that progress to skin tumours in old animals, but do not show any perturbated epidermal differentiation and stratum corneum formation (Yang et al, 2005; Qiao et al, 2006).
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