Therefore, the strategy of normalization and subtraction was used in library production.
Since we could not increase the precision of the immunomodulatory gene expression data with the reference genes, and we had alternatively controlled for appreciable amounts of systematic variability through study design, the strategy of normalization to reference genes was abandoned.
In addition to the normalization to total RNA input that was performed, a strategy of normalization to four reference genes as a function of average gene expression weighted by their variances was considered.
This process includes overcoming the resistance of the receiving culture or theory, which may be achieved by a number of strategies of "normalization" that Goffman, for instance, deftly demonstrates in his development of the concept of "face-work".
There are several strategies of normalization [8] but the most popular is the use of reference gene.
Collectively, these data identify a convenient new non-invasive marker for diagnosis, a strategy for normalization of tumors in vivo by control of resting potential, and a novel molecular pathway through which bioelectric events mediate morphostasis and the oncogene-driven escape of cells from the adaptive patterning program of the host organism.
As there are no endogenous miRNA established as normalizers for serum miRNA from women with or without SEOC, we assessed a number of candidates and compared these with serum volume normalization, the latter currently the most common strategy for normalization of serum miRNA [ 25, 26].
This study has identified a strategy for normalization of cardiac gene expression involving two reference genes, SRP14 and TPT1, that in combination may have broad application for accurate and reliable normalization of RT-qPCR data within failed human myocardium and non-failed human myocardium separately.
Six of these methods (GP-Theta, GP-Quantile, Global, Lowess, Quantile, and TMM) use different strategies of data normalization, but use the same DE tests as deGPS.
These conclusions were similar regardless of normalization strategy or over-dispersion estimation strategy (data not shown).
Currently, a number of normalization strategies for qPCR results exist, however, the use of reference genes is the most accepted one [ 3].
