To validate this strategy for isolating viable cells devoid of naturally occurring Treg, GITR−CD25−CD4+ cells were sorted from naïve CBA mice (Fig. 4A) and the resultant population stained for intracellular FOXP3.
Currently, there are no known specific surface antigens for SMCs that can be used as a strategy for isolating these cells via FACS.
Here, we describe a multimodal strategy for isolating pancreatic endocrine and exocrine cells from children and adults to identify age-dependent gene expression and chromatin changes on a genomic scale.
b, Sorting strategy for isolating neoantigen pool-reactive CD4+ T cells from peripheral blood ex vivo.
The strategy, named CCAST, for Clustering, Classification and Sorting Tree, identifies a gating strategy for isolating homogeneous subpopulations from a heterogeneous population of single cells using a data-derived decision tree representation that can be applied to cell sorting.
In particular, we isolated a series of complete human IgGs against human TFRC; these were generated using our novel strategy for isolating naïve antibodies against antigens with natural conformational epitopes on cell membranes via the phage-display method.
"We needed a comprehensive strategy for isolating the circulating DNA from blood and detecting the rare, cancer-associated mutations.
When West later learned that another university researcher, John Gearhart at Johns Hopkins, had made significant progress isolating cells very similar to embryonic stem cells, he headed straight for Baltimore.
In the longer term, approaches to identify surface markers and strategies for isolating healthy SP-like cells need to be developed to allow the clear identification of putative stem cells.
We systematically compared two strategies for isolating liver sinusoidal endothelial cells (LSEC) from mouse liver.
In this report we review current strategies for isolating, enriching, and, most recently, inducing the development of human pluripotent stem cells.
