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Recent sequencing based studies have illuminated increased transcriptional complexity within the genome structures of bacteria and modifications now allow use of strand specific sequencing which provides an accurate determination of the strand of origin of expressed regions of the genome [7], [8], [9], [10].
CGIs were classified into distinct classes related to their location relative to oocyte expressed transcripts for which the strand of origin could be determined by Cufflinks.
We did not observe correlations between changes in mitochondrial gene expression and respiratory chain complex, position on the mitochondrial genome or mitochondrial strand of origin (data not shown).
The changes in transcript levels that we observe upon inhibition of translation are not correlated with respiratory chain complex, position on the mitochondrial genome or mitochondrial strand of origin.
In addition, it was shown that the blocking structure derived from the paternal strand of origin is a valuable diagnostic tool to quantify and detect phasing and pedigree errors irrespective of the phasing method employed.
While RNA-seq is emerging as a powerful technology in transcriptome profiling, one significant shortcoming of the standard RNA-seq protocol is that it loses the strand of origin information for each transcript.
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While RNA-sequencing (RNA-seq) is becoming a powerful technology in transcriptome profiling, one significant shortcoming of the first-generation RNA-seq protocol is that it does not retain the strand specificity of origin for each transcript.
In the following initiation events, E. coli DnaB helicase is loaded, by interaction with DnaA initiator, to each strand of unwound origin DNA in the complex with DnaC protein (Fig. 1B) (Katayama et al. 2010; Kaguni 2011).
We focused on this genetic diversity and sequenced 130 Spirogyra small subunit nuclear ribosomal DNA (SSU rDNA) strands of different origin.
Figure 4A and B show the true and inferred strands of paternal origin (blocks) for 40 half-sibs and 10 000 markers (derived from dataset C).
To find indications on the location of the H-strand and L-strand origin of replication (OH and OL, respectively) in R. philippinarum mt genomes, we calculated the AT-skew values on 4-fold redundant sites of protein-coding genes, using the formula (A + T /(A – T).
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