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This has important implications in terms of low AC-loss strand design.
We consider hypercodes for involution codes, which are useful for DNA strand design, and define an involution hypercode.
We survey several formulations of the DNA strand design problem, along with results and open questions in this area.
Besides, more researches on the effect of strand design on critical current density and hysteresis loss for Nb3Sn strands are carried out.
DNA strand design also arises in use of molecular bar codes to manipulate and identify individual molecules in complex chemical libraries, and to attach molecules to DNA chips.
The possibilities of improving critical characteristics by optimization of strand design produced by the bronze and internal-tin process and by processing composite superconductors based on HTSC-compounds have been also considered.
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Quantum chemical modeling methods are used to propose candidate PNA Hoogsteen strand designs.
These include a DNA octahedron made from a long single strand designed to fold into the correct conformation, and a tetrahedron that can be produced from four DNA strands in a single step, pictured at the top of this article.
Depending on the organism and experimental system, the exogenous RNA may be a long strand designed to be cleaved by dicer, or short RNAs designed to serve as siRNA substrates.
The entire coding region of the TGM1 gene was PCR amplified using 0.4 μℳ of primer 5′-CTCCCTCCCACATAAGTCAC-3′, for strand and 5′-TAGCATCTGTTCCCCCAGTGCAAGTGAAG-3′, for strand, designed from the published cDNA sequences.
Primers amplifying the non-coding DNA strand designed with the aid of the BISPRIMER program [ 24] were as follows: forward primer 5'-GTTCGGATTCAAAAAAAGGGGT-3' and reverse primer 5'-CGATCATACCARCACTAAT-3'. The PCR program consisted on: one cycle at 94°C – 3 min; 35 cycles at 94°C – 20 s, 55°C – 20 s,72°C – 20 s; extension 72°C – 7 min.
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