Exact(11)
In our study, the sign of GC skew values on individual protein-coding genes was almost identical among analysis for four different partitions of gene sequences, indicating that deaminations occurring during replication, in addition to other mutations, contributed more than selection on amino acid sequences to the strand composition of G and C.
Strand asymmetry (also called strand composition bias) is a remarkable feature of animal mitochondrial genomes.
Hence, different Salmonella strains can be discriminated based on their sequence, length, GC content, or strand composition.
Strand composition of DNA depends on the role which the strand plays in the replication process – leading or lagging.
However, it is well known that the mutation process in the two DNA strands is different, leading to asymmetric strand composition called GC-skew [ 65].
The existence of two replicases implies a physical difference in the replication process that should be reflected in a bias in nucleotide strand composition.
Similar(49)
These findings show that deaminations during replication and other mutations contribute more than selection on amino acid sequences to strand compositions of G and C, and that the replication process has a stronger affect on A and T content than does transcription.
Indeed, upon spectral deconvolution using three combinations of algorithms and reference sets (17– 21), the MBP-ΔNCP69 structure is estimated to possess ∼1.4 1.8 times greater α-helical content and lower β-strand composition compared with MBP-ΔN597 (Fig. 3G).
Sense and antisense strand base compositions reported.
In fact, as noted in the discussion, it would be important to consider other compositional factors (such as the length distribution of overlaps, the frequencies of regulatory elements, and the strand-specific composition bias) as well.
A more complex compositional model for overlapping genes frequency, might include the length distribution of overlaps, the frequencies of regulatory elements (e.g., Shine-Delgarno sequences) and the strand-specific composition bias, since bacterial genomes have an asymmetrical chirochoric base composition [ 15- 17].
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