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The PAM sequence located 3 nucleotides upstream of the double strand break site is underlined.
The off-target mutation result in part from Cas9-mediated double strand break (DSB) of DNA.
Non-DSB clustered lesions comprise single strand break, base damage and abasic sites.
ND significantly enhanced double strand break accumulation at 24 h after ENU.
DSB = double strand break.
(D ) Kataegis localised to a double strand break.
Similar(42)
double-strand break.
single-strand break.
Ionizing radiation creates DNA damages such as double-strand break, single-strand break, and altered bases (Gavande et al. 2016).
where α expresses the ability of one particle to induce a DNA double-strand break.
Genome engineering with programmable nucleases depends on cellular responses to a targeted double-strand break (DSB).
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