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Crosses between strains were single burst lysates as previously described [ 23], but at a multiplicity of infection (MOI) of 10.
From 100 patient-control pairs, we isolated 78 HEp-2 adherent HEp-2 adherentestrainsstrains were single pathofens identhesed in stools of infants with diarrhea.
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Strains were single-colony purified and grown in the absence of antibiotics for experiments.
Regarding the leucocyte data, males of the SJL/J strain are singled out by enrichment in opposite trends.
Besides the anomalous clustering of some ' S. mitis' strains, there were single strains assigned as S. gordonii and S. infantis within the S. oralis cluster, a single strain of ' S. mitis biovar 2' within the S. anginosus cluster and a single strain of S. anginosus within the S. constellatus cluster.
Of the remaining 3994 4-NQO−induced mutations distributed across the genomes of 38 mutant strains, 3993 were single-nucleotide substitutions and one was a ∆G C single base-pair deletion (File S2).
The increase in Lineage 3 was due to an increase in the family CAS1-Kili, and most (44/49) of the CAS1-Kili strains were a single spoligotype (ST21).
Nucleotide differences in coding and 3'-untranslated (UT) regions of genes between the two strains were 3.0 single nucleotide polymorphisms (SNPs)/kb and 4.5 SNPs/kb, respectively [ 25].
"Those were easier to eradicate from the world because they were single strains of organisms -- both were viruses -- and they only infected one type of creature," Weinberg told HuffPost.
The M. aeruginosa strains used here were single-celled, small enough to be ingested, and did not contain microcystins [ 28, 29].
Transgenic strains were established from single G1 positive adults by backcrossing to wild-type (KIL strain).
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