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Five strains were not able to survive (>3 log reduction) 15 min in a medium acidified at pH 2.0 using the conventional testing method, but survival was improved significantly for some strains in the dynamic model.
Of note, 58.4% (52/89) of the F2 strains were not able to form tetrads at all, and the underlying incompatibility is not clear, partly because the sample size is too small.
These deletion mutant strains were not able to express pili on their surface (data not shown), yet the lack of pilus expression did not impact their ability to form biofilms on polystyrene plates (Figure 5B).
However, further investigation indicated that strains did not have the ability to utilize the polysaccharide A101 as carbon source, because strains were not able to grow in medium with A101 as the only carbon source (data not shown).
On the non fermentable substrate pyruvate, the HAP deletion strains were not able to grow.
Without a functional sortase, S. mutans strains were not able to perform several cell surface-related activities, including saliva-mediated adherence and aggregation [ 11, 24].
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We demonstrated that while ΔhrpB2+pUFR047_hrpB21-56, ΔhrpB2+ pUFR047_hrpB21-123 and ΔhrpB2+pUFR047_hrpB2T125A strains are not able to cause citrus canker, the truncated HrpB2XAC polypeptides and HrpB2XAC single amino acid substitution mutants are all however secreted to the extracellular space.
The F. noatunensis strains are not able to grow at 37DC and hence they are not virulent to human [ 5].
However, all IBDV strains are not able to replicate in cell cultures, so the DF-1 cells may not be adequate for all IBDV vaccine strains.
By contrast, Ab depletion by preadsorption of 11 antiprotein positive sera with both Mtb strains was not able to reduce the immunoreactivity against nonrecombinant protein).
But we noticed that in BW3110 and BL21 Rha- the optical density of the cultures increased significantly, though these strains are not able to use L-rhamnose as a carbon source, due to the lack of rhaB.
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