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pseudotuberculosis YPIII pIB102) [62] harboring the 70 kb virulence plasmid and isogenic mutant strains thereof (listed in Table 1).
Multiple species of bacteria and hundreds of strains thereof are being sequenced every year, thanks to cutting edge approaches such as re-sequencing wherein genome sequence of a reference organism is used as a scaffold to direct analysis of several different strains [1].
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—or some variant thereof.
For further comparative studies, the closely related S-layer-carrying (S+) strain G. stearothermophilus ATCC 12980 which is completely covered by the S-layer protein SbsC and the S-layer-deficient (S−) variant thereof was selected as model system (Egelseer et al., 1996).
To model this behavior using polycrystal plasticity, the critical stress to activate twinning (especially the strain hardening thereof) must be decreased.
A single band in gDNA of mutant strains and lack thereof in gDNA of the WT was considered representative of successful targeted disruption.
Briefly, bacterial cells were suspended in 10 mM MgSO4 at OD660 of 0.1 for the WCS strains and 10−2 dilutions thereof for the Pf-5 derivatives.
It is generally recognized that the structure and diversity of the human microbiome is of medical importance not only at the species level but also at the intra-species level given that bacterial strains or clonal variants thereof can differ greatly in virulence [ 1],[ 2].
The T. reesei (a) wild-type strain (blue bars) and the thereof derived err1 overexpression strain QPEC1 (red bars) as well as (b) Rut-C30 (blue bars) and the thereof derived err1 overexpression strain RPEC1 (red bars) were cultivated in shake flasks on D-xylose.
However, 3 Scottish strains (designated H1085 and variations thereof) were isolated from cat feces at a farm where a hemorrhagic colitis infection had occurred.
Escherichia coli strain DH5α was used for routine cloning and S. typhimurium strain SL1344 and isogenic mutants thereof, referred to by genotype, were used in this study.
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