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For both strains, growth with sodium butyrate as the substrate caused a shorter lag phase than with decanoic acid substrate.
By using various carbon sources (lactose, glucose, xylose, hemicellulosic hydrolysate) for strains growth, contrasted enzymatic activities were obtained.
The highest ME concentrations (0.117 0.195 mL/100 mL) combined with the lowest pH values (5.0 6.0) strongly reduced or even inhibited strains growth.
However, the comparison of antimicrobial data of plant extracts is very difficult, depends on the different factors, such bacterial strains, growth media, inoculum size, methods and the relative purity (Leouifoudi et al. 2015).
At that, the inhibitory effect of both pneumococci and pseudopneumococci on the M. catarrhalis strains growth should be probably attributed to the living cells of microorganisms, because of the treatment in chloroform vapors leads to the disappearance of this effect.
In the cases when the bottom agar layer stabbed with the test strains was not treated with catalase, we have observed very extensive, often merging zones of M. catarrhalis strains growth inhibition.
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Wells with no color change or strain growth, or with strain growth but no color change, were classified as containing unknown strains without P-solubilizing ability.
This difference in strain growth further increases with increase in age of concrete.
In conclusion, strain, growth medium and conditioning film all affect biofilm formation.
Finally, mutants and R-control strain growth rate were monitored by measuring dry cell weight (DCW) and OD595 values.
The culture stability of the strain growth on submerged fermentation medium was also examined at 45 °C for 48 h.
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