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Previous work on multiple SAR11 strains from a single population revealed one of the highest recombination rates recorded in bacteria [38], [39].
Unlike the MLSA data, the rarefaction curve of the coded CRISPR spacer arrays (each spacer represents a single character) shows that the diversity at the CRISPR loci is undersampled with 39 strains from a single hot spring (Figure S1B).
Recently, we extensively characterized a collection of such strains from a single city in Brazil (6 ).
ClonalFrame analysis also identified high number of recombining sites in the analyses that included multiple strains from a single species.
The number of strains from a single study site and belonging to the same PAC species is here defined as a population.
Previously, the genomes of 12 S. islandicus strains from a single hot spring in the Mutnovsky Volcano region of Kamchatka, Russia, were sequenced.
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A friend at the time blamed his death on the strain from a single decision he made following years of anti-communist badgering.
16SrDNA is more conservative than the wsp gene, and recombination between different Wolbachia strains may make it difficult to infer the type of strain from a single gene.
DNA was amplified for each strain from a single individual using the manufacturer's protocol of the REPLI-g kit (Qiagen), with the exception of the C. remanei strains from Ontario.
To date, most studies of gene expression variation within Drosophila species have been limited to a small number of laboratory strains, or to strains derived from a single non-African population [ 4- 8].
The utility of differences in explorative strategies and behavioral profiles among various outbred strains derived from a single strain is discussed.
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