By performing strain extraction using Continuous Wavelet Transform (CWT), the simulated interferograms are validated and matched against the FEA deformation field.
The production of recombinant proteins includes commonly processing steps such as development and culture of the strain, extraction, purification and formulation of the recombinant protein (Looser et al. 2015; Wolf and Reichl 2011).
Besides, in order to obtain the strain field from the fringe patterns, the linear fitting strain extraction method is proposed, which can achieve satisfying measurement accuracy and is easy for operation.
Using this wireless sensor, we further introduced a new telemetric monitoring modality that employs full electrical characteristics of the system to achieve correct strain extraction at any interrogation distance.
Therefore, at varying interrogation distances, it is not possible to attribute an individual resonance frequency value solely to an individual strain level, consequently resulting in discrepancies in the strain extraction if the interrogation distance is not kept fixed or distance-specific calibration is not used.
GW carried out isolation of the used strains, DNA extraction, PCRs, sequencing of meq and LTR genes and nucleotide alignments.
For molecular identification of the isolated strain, DNA extraction was done according to the methodology described by Raeder and Broda (Reader and Broda 1985).
For every strain, two extractions were performed and both extracts were measured in duplicate totalling of four spectra for each bacterial strain.
Genomic DNA was extracted from all K. pneumoniae strains (QIAGEN DNA extraction kit, QIAGEN, USA) and the rmpA and serotype-specific genes for the K1, and K2 capsular serotypes were amplified by polymerase chain reaction (PCR) as previously reported [ 16, 17].
