Using our evolved strain, efficient fermentation with reduced inoculum sizes and shortened process times was possible, thereby improving the overall economic viability of a woody biomass-to-ethanol conversion process.
In yeasts, they have been applied to analyze the genetic variation within and between wild and domestic populations [ 11], the spectrum of spontaneous mutations in Saccharomyces cerevisiae [ 12] and the adaptive mutations in a Pichia stipilis strain efficient in xylose fermentation [ 13].
These strains exhibited efficient fatty acid production when xylose was used as the sole carbon source.
Moreover, we show that in NHEJ deficient strains, highly efficient marker-free gene targeting can be performed.
The ability to engineer industrial strains for efficient sucrose utilization will allow substitution of sucrose for glucose in industrial fermentations.
Furthermore, emphasis is given on the latter advances in engineering S. cerevisiae strains for efficient whey-to-ethanol bioprocesses.
These strains allow efficient labeling of aromatic amino acids in recombinant proteins, supporting NMR structural biology and a wide range of other biophysical studies.
These strains displayed efficient growth and ethanol production from biologically pretreated rice straw hydrolysates.
Moreover, this review provided perspectives and guidelines for future developments of robust strains for efficient bioethanol or biochemical production from lignocellulosic materials.
