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Both strains are in a white-eyed pearl mutant background to facilitate detection of eye-specific fluorescence.
These observations underscore the need to focus prevention efforts on the short window of time after passage of feces when strains are in a hyperinfectious state.
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The fact that two sonnei strains are grouped with three EIEC strains supports the hypothesis that EIEC strains are in an intermediate stage and are a potential precursor of "full-blown" Shigella strains [ 22].
All the strains were in a mixed genetic background.
None of the XDR strains were in a genotype cluster.
This suggests that the reducing equivalent produced by these two strains is in a similar amount.
The resulting double-null mutation was confirmed by PCR genotyping (supplementary Fig S3A; both mouse strains were in a C57/BL6 genetic background).
The ultimate approach to compare the capacity to establish infection of the 3 strains is in an in vivo environment.
The sequence variations between the two strains were in an antigenic important part of the G protein.
As a result, assembly intermediates from factor-null strains are in principle a heterogeneous set of presumably related intermediates.
Those strains are in part a by-product of the 2010 dispute over the Gaza flotilla.
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