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To monitor rRNA levels, 10 μg of total RNA from each strain was resolved on a 1.2% formaldehyde/agarose gel.
Promoters were predicted by "Neural Network Promoter Prediction" software Approximately 300 ng undigested planarian genomic DNA from each strain was resolved on agarose gels and transferred to Hybond-N membranes (Amersham).
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After an initial surge in aggregate formation, which occurred to a similar extent in both wt and rpt4-145 cells upon the heat stress, aggregates formed in the wt strain were resolved at a markedly higher rate (Fig 2F& G).
Similarly, group D had extensive radiation of ITS2 variants and no relationships among strains were resolved (Additional file 4).
Within Prochlorococcus, relationships among strains are resolved almost congruently by rbcL and rDNA data: MIT9313 appears as the basal divergence, followed by strains NATL2A, CCMP1375 and MIT9213 (relationships among these strains are not resolved), and a derived, long-branched clade consisting of MIT9312 and CCMP1986.
The remaining 10 mammal-infecting Lagenidium strains were resolved with strong support into 3 independent clades that are basal to L. giganteum and identified in this study as L. ajelloi, L. albertoi, and L. vilelae.
Deletion strain abundance was resolved by averaging scanned downtag and uptag intensities for each strain and comparing intensities between the tunicamycin-treated pool and the DMSO-treated pool [ 14] (see Additional File 1).
One prior study reported that tamoxifen induction of the αMHC-MerCreMer strain induces transient myocardial dysfunction, which was resolved after 2 weeks (Koitabashi et al., 2009).
Where the strains analysed were more numerous, particularly in lineage IV, the phylogeny was resolved into clusters corresponding to strains with the same regional localisation.
Discrepancies in start coordinates between homologous genes in strain KN400 and strain PCA were resolved by identifying plausible ribosome-binding sites.
But little was resolved.
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