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Single colony of each transformants and mutant strain was dissolved in 100 μl sterile water.
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Two µg of DNA from each strain were dissolved to 80 µl MilliQ water and sonicated to 500 2000 bp by digital sonifier (Branson Corporation, Danbury, CT).
Serogroup B CPS purified from a LOS-deficient meningococcal lpxA strain were dissolved in sterile H2O at 1 mg/ml concentration and 20 µl were transferred into an Eppendorf tube mixed with 10 µl (5 µg) of LL-37, 10 µl (0.5 µg) of human rCD14 and 10 µl (0.5 µg) of rLBP (R&D Systems) in 30 µl final volume and incubated for 3 h at 37°C.
Thus, the biofilm mass of all tested strains was dissolved with SDS and measured.
One mg of biliverdin producd by strain E. coli strain BL21 mHO1) was dissolved in 2mL methanol, and 0.2mL was mixed with 1mL of the kit assay buffer.
A commercial LPS preparation (Sigma Escherichia coli strain O55 B5) was dissolved in water and applied to cells in culture media at a final concentration of 10 μg/mL (Boutros et al. 2002; Horn et al. 2010).
Rifampicin was dissolved in DMSO and used as positive control drug and extracts/drug free medium with strain suspensions were used as negative control.
His contract was dissolved.
Arthur Andersen was dissolved.
(It was dissolved in 2007).
His Thai Rak Thai party was dissolved.
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