The CMCase activity of the other 19 strains was undetectable after cultivating in various liquid media for up to 6 days, and the Avicelase, FPase, and β-glucosidase activities of all the 22 bacterial strains were also undetectable.
Since 10-10 g of PrP from both prion strains was undetectable in either immunoblot or ThT-fluorescence, we chose it as the initial seed.
The kcs1Δ yeast strains had undetectable levels of IP7 and IP8 [ 13, 14], and displayed slow growth, temperature sensitivity [ 13, 15], an impaired stress response [ 13, 16] and altered cellular energy dynamics [ 17].
In a csgG− strain, CsgF is undetectable from whole-cell samples, but neither of the curli fibre subunits CsgA and CsgB nor the accessory protein CsgE was required for CsgF localization at the cell surface (Nenninger, Robinson and Hultgren 2009).
Transcript levels for trkB do not differ appreciably among halAΔ, sltAΔ, halAΔ sltAΔ and wild type strains (Fig. 5) and transcript levels for nhaA in these strains are virtually undetectable.
Genetic screens for IHHNV may allow the identification of otherwise undetectable strains, as well as shed light on the unknown determinants of changes in virulence.
Furthermore, both BSW3AP and BSW3AG produced small amounts of glycerol (1.4 g L−1 for both strains) and almost undetectable amounts of arabitol and acetate.
Finally, it should be noted that potentially additional metal resistance determinants could be present in the other strains, which are undetectable with a CGH array based on the CH34 genome.
While the small DNA pieces are also present in a number of strains with mutations in predicted nucleases, they are undetectable in strains containing mutations in nuc-1, which encodes a DNase II endonuclease.
CF derived from this mutant strain shows low to undetectable levels of protease activity.
