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Strain the solution to remove the seeds and the pulp.
Strain the solution through a coffee filter to remove any impurities.
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Any remaining pieces of tissue were removed by straining the solution through a nytex filter, and the cells in suspension were centrifuged and resuspended in DMEM + 5% FCS.
Three essential components of the ERM, i.e. the residual strain measurement, the solution of the inverse problem of eigenstrain theory, and the Simple Triangle (SIMTRI) method, are described.
The effect of finite inelastic strains on the solution behavior of the PDASS method is examined in detail based on the complementarity parameters.
For each strain, the four solutions were pooled and the DNA precipitation and washing steps were repeated.
The constitutive equation, which contains derivatives of both stress and strain, significantly complicates the solution to the problem.
Two different responses have been observed: strain thinning in the melt state and weak strain overshoot in the solution state.
Moreover, based on the definition of cumulative plastic strain κ s, the solution with "plus" discriminant is adopted to determine the cumulative plastic strain corresponding to the peak stress (i.e., the nominal CPB strength).
It was demonstrated that the sensitivity to strain and chloride solution can be enhanced by CNF.
By using a constraints-based flux analysis on a genome-wide reconstructed metabolic model of the engineered strain, we obtain the solution space within cell's capacity.
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