A barcode-tagged deletion strain set for S. pombe, in which each deletion mutant carries two barcodes, has also been developed recently [ 17].
We hypothesized that certain EAEC lineages predominate among isolates from childhood diarrhea and that hypervirulent lineages would be associated with diarrhea in a strain set from a controlled study.
Deletion of DBF2 and SLI15 in the nonessential strain set resulted in strains that produced a low level of blebbing.
In order to identify yeast strains that may be resistant to contaminating furfural, the SGRP strain set described in methods was grown in YNB, 100 mM glucose and the presence of 1.5 mg ml−1 furfural.
Presumably, the transmissible plasmids identified in our strain set play a major role in the spread of virulence and resistance genes among S. Heidelberg and, most likely, other Salmonella serovars.
In our validation strain set, we chose 28 strains in GenBank as of 29 March 2010 (Additional File 1, Table S1) that had extensive (>95%) alignment between Segments 3 and 4. We limited the analysis of Group E strains because there is negligible recombination of backbone DNA between members of this clade [ 39].
The population structure of P. aeruginosa in this strain set based on MLVA9-Utrecht is characterized by a high level of host-specificity.
The species- and sublineage-specific polymorphisms examined in this study for the M. africanum clades may therefore be of benefit when characterizing the evolutionary history of MTC strain sets in the future.
Development of the RI strain set utilized gender reciprocal crossing, providing two strain sets that differ in the source of mitochondrial DNA and the Y chromosome.
Though The Strain is set in New York, the combination of a generous tax credits system and its "could be anywhere" architecture means Toronto is often used as a location for shows based elsewhere.
Eye strain can set in and cause blurred vision, headaches, dry eyes and pain in the eyes.
