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The exploitation of dynamic process conditions increasingly finds attention for strain screening and bioprocess development of biotechnological products.
Meanwhile metabolite-responsive transcription factors and riboswitches have been implemented in strain screening and evolution, and in dynamic gene regulation.
To promote the application of microfluidic-based technologies in strain improvement, this review addressed the potentials of microfluidics and the related approaches in DNA assembly, transformation, strain screening, genotyping and phenotyping, and highlighted their adaptations for single-cell analysis.
Due to its advantages, this method can help in strain screening and designing of proper strategies for maximal biomass development, formation of rare sugars as well as general utilization in the emerging field of algal biorefinery.
For high-throughput strain screening experiments, the parent strain of L. paracasei NCBIO01 was adopted.
Meanwhile, parallel micro-bioreactor system could well meet the screening flux requirement in the process of high-throughput strain screening.
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Li and other researchers successfully isolated the GA-producing fungal strain, screened high-yield strains, and optimized fermentation conditions with the guidance of Prof. Dafu Yu (Dafu et al., 1964).
For example, an ipaD mutant, in which effector secretion is constitutive (Menard et al. 1994), was the most Congo red−positive strain screened.
The optimization of rhamnolipid production with P. aeruginosa strains, screening methods for new non-pathogenic natural rhamnolipid producers and recombinant rhamnolipid production are examined.
Various strategies for microbial production of 1,3-PD from glycerol including strains screening and improvement, two-step and multi-stage fermentation, sole and co-substrate fermentation, co-culture, and microbial consortium were reviewed and compared.
Both literature and internal intra-laboratory assessments of the test, at least in a 2-strain screening version of the test, have found discrepancies on the order of 15-20% [9].
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