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Its reactivation in an avirulent strain restored a virulent phenotype [34].
Furthermore, plasmid-mediated expression of rgfAC in L1ΔrgfAC mutant strain restored its fibrinogen-binding ability to the wild-type level.
Complementation with the wildtype Fun30 in the fun30-deleted strain restored the silencing of the ADE2 reporter embedded in the HMR (Figure 9A).
Introduction of exogenous expression of the rovA gene under the control of the arabinose-inducible promoter araBADp into the mutant strain restored RovA expression when induced with arabinose.
Co-expression of the derivatives with PcrV in the parental PA103 strain restored hemolytic activity to the levels of positive control strains (filled bars, Fig. 7B).
Introduction of a plasmid copy of the revertant luxS gene into the 81116AI2 -strain restored its ability to produce AI-2 to the same level as the 81116AI2+ strain (Figure 1B).
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In summary, these observations suggest that the overexpression of either phaZ1 or phaZ2 in this strain restore the ability of Re2005 to utilize PHB under the given conditions, whereas the overexpression of phaZ3 and phaZ5 appears to have no effect.
The method is validated through the identification of the highly nonlinear hysteretic behavior produced by the experimental setup described in Tasbihgoo et al. (2007) [2] involving displacement and strain (restoring force) sensor readings.
Interestingly, when these repeats (as much as a 158-residue central stretch) are deleted from MamJ, mutant forms are still able to complement a M. gryphiswaldense MSR-1 ΔmamJ strain, restoring magnetosome alignment with the MamK cytoskeleton [22].
Simultaneous expression of wild-type P. fluorescens LapD and LapG in the double-deletion strain restores the phenotype of the parent strain with native LapDG levels, characterized by a low-to-intermediate biofilm mass.
Here, we show that domestication can be reversed when a domesticated strain is challenged by various adverse conditions; the resulting feral strain restores its ability to form structured biofilm colonies.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com