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Table 1 The effect of Spermine NONOate and MAHMA NONOate on preformed biofilm Probability > F (p = 0.05)* Material Strain Incubation temperature Spermine NONOate MAHMA NONOate Polypropylene Salmonella 14028 4°C n.s.s
Similar to the observations in the GPA1/Gαs strain, incubation with increasing concentrations of the antagonist resulted in a rightward shift of the GLP-1 dose-response curves decreasing the potency and maximal responses demonstrating an insurmountable effect.
Zygote selection, presporulation, and sporulation were performed as described previously with the exception that strains were incubated at RT. Haploid and double mutant selection were as described previously with strain incubation being carried out at RT for 2 d.
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Hatched larvae were collected over a 24 hr period by straining incubation tank water through a 90 μm sieve and fixed in 95% EtOH.
The discrepancies between MIF and ELISA in the detection of anti-Cpn IgA antibodies may have been due to the different experimental conditions used in the two serological tests, including the Cpn strains, incubation times, antigen concentrations, serum dilutions, absorption experiments and the types of conjugates used.
To determine if initiation of chromosome replication had occurred in the dnaC2 strain during incubation at the non-permissive temperature, cells were incubated for 90 minutes at 38°C and 300 µg/ml rifampicin and 10 µg/ml cephalexin added to the culture to prevent further initiation and cell division, respectively.
To test whether Opa loop-specific antibodies can block gonococcal interactions with human CEACAM-expressing endocervical cells, ME180 cells were inoculated with the constitutive OpaB-expressing strain following incubation with AbHV2BD linear, AbHV2BD cyclic, or AbSV cyclic.
A mean ratio of cells per 0.1 mm² was then calculated for each strain and incubation type (d- versus l-leucine).
As a control, we used heat-killed bacteria of strain Db11 (incubation at 70°C for 15 min).
Among seven amino acids studied, serine is the first one to be consumed from a wt strain during incubation in a complex medium similar to LB [ 28].
Sensitivity was determined by comparison of growth with that of the wild-type strain after incubation at 28°C for three days.
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