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The development of a multi-sample strain device and elastomeric culture wells designed to systematically assess strain effects on cell cultures is presented in this report.
This study examined the effects of mechanical strain on osteogenic and adipogenic differentiation of cultured MSCs by stimulating MSCs cultured in general and adipogenic differentiation media using a mechanical strain device.
Additional FRET calibration of Fn embedded in fibers was performed via a custom-made strain device and used to correlate Fn fiber FRET ratios with fiber uniaxial strain, as described in Refs. [ 27, 28].
Markers of osteoblast differentiation (Runx2, Osx, and I-collagen) and adipogenic differentiation (PPAR γ-2, C/EBP α, and lipid droplets) were observed in MSCs stimulated by a mechanical strain device, cultured in both general and adipogenic differentiation media in vitro.
AF cells were isolated from nondegenerative and degenerative human IVDs, expanded in monolayers and cyclically strained in the presence or absence of the cytokine inhibitors IL-1 receptor antagonist (IL-1Ra) or IL-4 receptor antibody (IL-4RAb) with 10% strain at 1.0 Hz for 20 minutes using a Flexcell strain device.
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Vertical strips were clamped in the stress-strain device.
Strain pulp through fine muslin or another fine straining device, wringing the material dry.
Do not use a straining device when transferring soaked clams as the sand and other particles will wash over the clams again from the bottom of the bowl.
Data were collected over nine decades of shear rate, using controlled strain and controlled stress devices, steady shear and oscillatory modes, rotational rheometry, and capillary rheometry using the multi-pass technique.
The impact of strain on device performance is shown to be well modeled using the classic bulk-Si piezoresistance (PR) coefficients.
Therefore, a two-dimensional strain measuring device is realized, which is capable of measuring large angle strains.
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