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Rescue strain construct concentrations are listed in Table 1 with each being co-injected with the pmyo-3::CFP marker (L4816; Fire Lab C. elegans Vector Kit, 1999 plate) (20 ng/µl) and pCR2.1 added to a final injection concentration of 100 ng/µl.
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The recombinant strain constructed here has great potential for large-scale production of active trypsin.
The ku80 null strain constructed using the sulfonylurea resistance cassette was designated HLS1002.
The CreA::GFP strain constructed in the present work, demonstrated growth similar to the parental strain on various repressing and derepressing carbon sources (Additional file 1: Figure S1).
By contrast, an isogenic hypermutable strain constructed from the wild-type P. fluorescens evolved resistance to all treatments regardless of immigration, but typically suffered very large fitness costs.
Recombinant Saccharomyces cerevisiae strains constructed by metabolic engineering approaches can ferment xylose but with low efficiency.
The strains harboring cocoa genes were constructed based on S. cerevisiae CEN.PK 113-11C all all the yeast strains constructed in this study are listed in Table 1.
The robust strains constructed in this study can be applied for producing chemicals and advanced biofuels from cellulosic hydrolysates.
All of the transgenic C. elegans strains constructed in this study are listed in Table S1.
Primers used are listed in Table S1; strains constructed are listed in Table S2 in the Supporting Information.
The resulting S. cerevisiae strains constructed in this study are listed in Table 2, with their respective genotypes.
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