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These results represent an important technical advancement for the molecular biological investigation of truffles, which will be instrumental to the construction of mutant strain collections.
Efforts are underway to link mutations with drug resistance and to develop strain collections to enable assessment of new diagnostic assays.
In this work we used a genome-wide screening of S. cerevisiae diploid mutant strain collections to identify genes that potentially contribute to adaptation to low temperature fermentation conditions.
Large numbers of SNPs have been identified and used to genotype worldwide strain collections.
For example, yeast mutant strain collections has become increasingly used to identify the phenotypes [17].
Previous investigations were done on Y. pestis strain collections representing only a limited genetic diversity of the species.
For research, relatively few cpe-positive type C strains are available from strain collections in Japan, USA, and Europe.
The availability of yeast strain collections expressing individually tagged proteins to facilitate one-step purification provides a powerful approach to identify proteins with particular biochemical activities.
Clinical isolates of E. aerogenes belonging to two strain collections isolated in 1995 and 2003 respectively, were screened to assess the involvement of efflux pumps in antibiotic resistance.
Equivalent diversity in plasticity zone gene content had also been found by DNA hybridization in Costa Rican and Mexican strain collections [28], [29].
These findings caution that selection of molecular markers for epidemiological typing should be based on large and globally representative strain collections.
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