These assays have a straightforward, quantitative readout: animals that regenerate a functional pharynx are able to ingest food (dyed to monitor food intake).
The two-step screening procedure consisting of an IgG-ELISA followed by IFA of transfected cells was quick, and the readout straightforward.
With low input capacitance per channel (10 100 fF) it is relatively straightforward to design pulse processing readout electronics with input referred noise of ∼100 e-rms and pulse shaping times consistent with tagging of events to a single LHC bunch crossing providing clean 'images' of the ionising tracks generated.
The use of fluorescein-biotin enabled straightforward comparison with the fluorescence readout demonstrated previously.
Such core screening facilities may work on straightforward screening projects with routine assays or readouts, or, as in our case, may be required to conduct many diverse projects simultaneously using a wide variety of assays and cell lines.
Although this appears to be a straightforward procedure, standard addition approaches require a nondestructive readout because the sample is spiked and the signal measured, and then an additional aliquot of standard is added and the sample reanalyzed.
Following the anticipated mode of action of the individualised vaccines, the induction of functional mutation-specific T-cell responses, as well as their trafficking to the tumour as determined by functional immunological readouts, and TCR repertoire profiling are straightforward strategies to achieve the clinical proof-of-concept first in human trials.
In summary, FC calculated from intracranial electroencephalography works as a readout of brain functioning and provides a straightforward measure for studying the effect of biological alterations occurring at the molecular, cellular and physiological scales during the transition from a healthy brain into an epileptic one.
Short-term or long-term readouts like heartbeat, mechanical contraction, viability, hERG activity, or Ca2+ fluxes are easy and straightforward to measure, thereby offering a sensitive model to assess cardiac toxicity.
It became, therefore, straightforward to predict the recombinase enzymatic activity at any time without the need to wait for the morphological/physiological readout of the treated cells.
