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Here, we present an alternative Escherichia coli-based open cell-free synthesis (OCFS) system that is optimized for predictable high-yield protein synthesis and folding at any scale with straightforward downstream purification processes.
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However, these contributions have not addressed the effects of RAS mutation on downstream effectors, which are not as straightforward as simply enhanced effector activities.
Downstream of this point lean principles are applicable in a straightforward manner, while upstream lean needs to be interpreted in a more liberal way.
In this paper we have introduced a general definition of gap metabolite that allows its detection in a straightforward manner, even for the cases of upstream-non-produced and downstream-non-consumed metabolites.
There is no straightforward method to confirm functional inhibition of NKG2D as this receptor has multiple downstream targets that are shared with other pathways.
The most straightforward explanation for this difference in actin tail length is that Grb2, which is downstream of phosphorylated tyrosine 132 of A36, can interact with WIP, but not WIRE.
However, the functional implication of this difference is not straightforward because the RNA-recognition motif of the Dazl protein is located 30 amino acids downstream and is not affected, unless induced by configuration change.
Downstream, the canyon deepened.
Upstream or downstream first?
I looked downstream.
People downstream were evacuated.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com