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Straightforward assembly of the devices was designed by means of using a UV photo-curable gel.
To overcome many limitations of chemical cross-linking methodologies for preparation of bispecific antibodies, we have employed heterobifunctional PrA-PEG-SA adaptors for straightforward assembly of unmodified anti-Her2 and biotinylated anti-CD3 antibodies into bispecific CD3xHer2 targeting ligands.
For the straightforward assembly of the (1→8 -linked disaccharide units, the peracetylated 8- O- tert-butyldimethylsilyl allyl glycoside derivatives of Kdo (1→8 -linked(14) were chosen as glycosyl acceptors that allow disaccharideing with flunitse donors and provide access thethe resperacetylatedol by reaction with fluoride ions.
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Cermak et al.[ 13] have recently established a particularly straightforward TALEN assembly system that is based on GoldenGate cloning.
Despite the straightforward in vitro assembly, the assembly of the Fe S clusters in vivo is a more precise and complex process.
During the war entire new factory cities were organized and built with the straightforward efficiency of assembly lines.
Using vectors of pHellsgate proved to be a straightforward method for assembly by Gateway recombination of hpRNA constructs, designed for high-throughput gene silencing in plant cells [21].
Although these elements allow for straightforward and rapid assembly of synthetic devices, they may not be optimal for the design of sophisticated transcriptional networks that rely on endogenous genetic elements or coregulatory factors.
In general, incorporation of two binary affinity systems, Protein A G, L /Antibody and biotin/streptavidin, within a single adaptor construct offers a straightforward route for assembly of target-specific antibodies with other functional biotinylated ligands, such as antibodies, reporter molecules, and therapeutic moieties, without requiring extensive customization.
The notions of contig and singleton are straightforward for perfect assemblies: a contig is any sequence produced by two or more overlapping reads, while singletons are the remaining isolated reads.
Supramolecular assembly allows straightforward tuning of the density of functional BTA monomers within these BTA polymers and previous work has also shown that the distribution of functional monomers can be externally controlled, e.g., via interaction with multivalent templates32.
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