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Quadruplex priming amplification (QPA) is a straightforward assay that allows isothermal amplification of DNA and possesses an intrinsic real-time detection mechanism.
This is a straightforward assay to monitor responses of human vascular endothelial cells.
We therefore utilized this straightforward assay to evalute the effects of compounds 1– 17 on A. thaliana seedlings.
The criteria for a useful teratogenicity screening should include cost-effectiveness, adequate throughput, straightforward assay conduct, reproducibility, and concordance with in vivo mammalian data [ 4, 6].
The fluorescence quenching of biotin-4-fluorscein (B4F) provides a straightforward assay to quantify the number of biotin binding sites per SAv QD.
Therefore, we pursued the development of a straightforward assay that would indicate binding of small molecules in the active site of HK853, with the ultimate goal of validating the identified leads with multiple HKs.
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This review outlines a detailed protocol for purification of native Cre and describes straightforward assays that can be used to test for recombination activity in vitro.
They capitalize on femtomolar sensitivity, profiling full proteomes and high-throughput yet straightforward assays.
In this present study, we have validated two relatively straightforward assays that could be used: firstly, the recruitment of GFP-tagged XPC proteins to 408 nm laser damage, an assay which is appropriate for the evaluation of a range of DNA repair genes, and secondly, the 3′ UTR assay which is generic and so could be applied to any gene.
Both protocols might be useful for high-throughput ChIP-based assays, which are expensive, labor intensive, require complex statistical analysis, and might greatly benefit from faster and straightforward ChIP assay and analysis methodologies.
Although the different purified complexes were found to have differences in stability, and in their sensitivity to low concentrations of SDS and salt, the results suggest that it is not straightforward to assay selectively for each type of complex in cell extracts.
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