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Historically, plasmalogen molecular species have been separated by straight phase or cation exchange high performance liquid chromatography (HPLC) into lipid classes and subsequently analyzed by reversed phase HPLC [12].
After irradiation the provitamin D4 ampoule was dried with N2 gas and prepared for straight phase HPLC.
UV irradiated mushrooms revealed the presence of previtamin D4 with a retention time of 6.2 min in straight phase; its presence was confirmed by chromatography after its conversion to vitamin D4.
It is important to realise that straight phase trajectories, regardless of the saccade kinematics, are a consequence of the idea that the SC is directly involved in the motor program of the saccadic eye movement.
An aliquot of the organic phase (50 μl) was submitted to straight phase HPLC (Waters, Millipore, St Quentin-Yvelines, Quentin-Yvelines, Francex-SIL colusing4.6·250 mm, 5 μm particle siZorbaxwlett Packard, Les Ullis, France), isocolumn elution with 70/30/0.25 (v/v/v) hexane/diethyl ether/acetic acid at a flow rate of 1.5 ml min-1, and UV detection at 234.6·250
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Hydroxy fatty acids were separated by straight phase-HPLC (SP-HPLC) using a LiChrospher® 100 Diol column (Merck, Darmstadt, Germany; 125×4 mm, 5 µm particle size).
The fraction of interest was collected and then injected on straight-phase column, to separate the 13-HPOT and 13-HOT.
Each iteration of the honeybee waggle dance consists of a straight waggle phase, whose duration indicates distance to the food source, and whose direction relative to gravity encodes the direction of food relative to the sun's azimuth [1] [3].
If the vaccines prove safe and able to trigger the desired immune responses, an earlier WHO consultation found wide support for shortcutting the standard progression of vaccine clinical trials and jumping straight from these phase I trials to phase III efficacy tests in Liberia, Guinea, and Sierra Leone, the three hard-hit countries.
Last fall, an international ethical panel convened by the World Health Organization (WHO) judged that the unprecedented threat made it acceptable to take Ebola vaccines straight from small, phase I safety studies to large phase III efficacy studies, while simultaneously conducting phase II studies.
However, second, we found that cells taken straight from stationary phase and diluted to an OD of 0.3 (the density at which early log-phase cells were subjected to TLD) showed similar or greater sensitivity to TLD than cells in early log phase (supporting information, Figure S1).
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