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Although assessing differences in gene expression is relatively straight forward, analysis of cell type specific alternative splicing often is more complicated.
In contrast to the more straight forward analysis of the in vitro data, the in vivo data can therefore be analyzed in two different ways, as described in the Methods.
Although different theoretical approaches tried to analyse the effect of decreasing worker productivity within the framework of optimal dynamic resource allocation, the most straight forward analysis has never been performed: replacing the linear dependence of resource allocation on worker number (see eqns. 1 and 2) by a logistic relationship.
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For ChIP-Chip data, a straight-forward analysis of reproducibility is difficult.
Several factors impede a straight-forward analysis of microarray database content: standards for data submission vary between different databases, some microarray datasets do not provide raw data and on the experimental side, protocols and experimental conditions can differ between diverse laboratories conducting microarray hybridizations.
A simple library preparation procedure, rapid sequencing and straight forward bioinformatics analysis enable this efficient workflow.
A deletion analysis of TPRs in a model system such as budding yeast [Fillingham et al. unpublished observations] that is amenable to a relatively straight forward biochemical analysis may provide further insights into specific TPR motif function.
Analysis B is a straight forward case-crossover analysis falsely assuming that the risk of falling is equal over the whole day.
Some groups certainly must worry about alternate splicing, however most groups would find what they are looking for by performing a much more straight forward gene level analysis.
However, the anonymous and dominant nature of DArT markers do not allow straight forward comparative map analysis or identification of candidate genes associated with QTL.
One of the drawbacks of the technique is that the interpretation of the results is less straight forward than for ROI analysis.
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