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At least a dozen franchisees have sued, alleging they were stripped of their 7-eleven businesses and that stores were then flipped to new franchisees willing to pay higher fees.
The non-mitochondrial Ca2+ stores were then loaded for 45 min in 120 mM KCl, 30 mM imidazole-HCl (pH 6.8), 5 mM MgCl2, 5 mM ATP, 0.44 mM EGTA, 10 mM NaN3 to prevent mitochondrial Ca2+ uptake, and 150 nM free Ca2+ (28 μCi/ml).
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The effect of IP3 on Ca2+ release from the intracellular stores was then assessed.
That NAADP did indeed mobilize Ca2+ from acidic lysosome-related stores was then confirmed in a variety of mammalian cell types (16, 47, 69, 79, 98, 105, 108).
The 8mm reels on which the film was stored were then nailed into the heels of their makeshift shoes.
The humidification system in a chicory root cold store was then optimized.
All the activity data stored is then associated to the current learner id and is not anonymous.
The store was then loaded with merchandise obtained from restaurant supply houses and cooperative food manufactures.
All spores were then stored in darkness until use.
This stock was then stored at −80 °C.
Samples were then stored overnight at 4°C.
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CEO of Professional Science Editing for Scientists @ prosciediting.com