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However, under conditions of more severe stress, resulting in abolition of primary energy stores and cell death, the pHe continues to decline in the absence of a corresponding accumulation of extracellular lactate.
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These findings may be explained by the release of intracellular glutamate stores and cell-membrane glycerol by cell destruction.
After a total of 24 h incubation, culture supernatants were removed and stored and cell lysates were collected.
Currently, cryopreservation is the most reliable and established technology to store tissues and cells.
The only clues to Fischer's disappearance was bank records that indicated she had stopped at a store in Newport, Tenn., and cell phone records that indicated her phone had pinged a tower Thursday, along N.C.
The BAL fluids were aliquoted and stored at −80°C, and cell pellets were resuspended in 500 µl of Trizol (Invitrogen Life Technologies, Carlsbad CA).
In contrast, internal calcium can be divided into two compartments: internal calcium stores and calcium in cell plasma.
The device will be able to wirelessly download and store data from PCs and cell phones.
ROS damage biomolecules and cause oxidative stress by depleting cellular antioxidant stores and may result in cell death [ 6- 8].
Cells were washed twice with 5 mL PBS and cell pellets stored at −70 °C until cell extraction.
RNAprotect-treated cells were centrifuged and cell pellets stored at −80 °C.
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