For storage, specimens were transferred sequentially into 50%, 80%, and finally 100% glycerol.
Following 2 3 days of storage, specimens were rinsed in PBS, postfixed in cacodylate-buffered 1% osmium tetroxide, dehydrated in an ethanol series, and embedded in polybed 812.
For low permeable geomaterials such as clay shales, core extraction, handling, storage, and specimen preparation can lead to a reduction in the degree of saturation and the effective stress state in the specimen prior to testing remains uncertain.
After storage, each specimen was submitted to the microshear test on a universal testing machine (Kratos LKC3, USB, Brazil).
In spite of advanced expertise in fibrooptic upper tract endoscopy, issues with ureteroscopic biopsies such as suboptimal tissue volume, poor quality, lack of standardisation of biopsy technique, and type of preservative for storage of specimen remain unresolved.
The main challenges are storage of specimens and the labor involved in retrieving specimens for further testing [ 1, 5].
After 1-year storage, all specimens appeared more prone to interface detachment during specimen processing, rendering proper interface characterization more difficult.
After storage, the specimens were rinsed with tap water and dissolved in nitric acid.
On the other hand, energy storage for specimens deformed at Tg results to be practically independent from the loading rate.
A vast volumetric swelling up to 40%, and thus a huge reduction of the bending strength, was observed during the storage of specimens in PBS.
