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An acidic stopping solution was then added, and the enzymatic turnover rate of the substrate was determined by dual wavelength absorbance measured at 450 and 620 nm.
An acidic stopping solution was then added, and the degree of enzymatic turnover of the substrate was determined by a dual-wavelength absorbance measurement at 450 and 620 nm.
One hundred microliters of stopping solution was then added into wells, these were shaken for 10 seconds, and measured within 10 minutes using an ELISA reader at a wavelength of 450 nm.
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100 µl ANTS stop solution was then added to each well and the emission was measured at 405 nm.
Stop solution was then added to each well.
Stop solution was then added to each well and mixed.
DSN stop solution was then added, and the sample was incubated for 5 minutes at 70°C.
Stop solution was then added to each well and albumin concentrations were measured colorimetrically.
The Stop Solution was, then, added and mixed by a vortex.
Lastly, 50 μL of stop solution was then added into the wells and absorbance of each of the well was read with a plate reader at 490 nm.
The stop solution was then added to each well and the absorbance was read at 450 nm using a microplate reader.
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