Sentence examples for stopping solution was added from inspiring English sources

Finally, the stopping solution was added and the optical density was read spectrophotometrically at 450 nm.

After incubation for 30 min, the wells were extensively washed for three times, followed by the addition of 100  μL trimethylbenzene solution and incubation for 30 min before 100  μL of stopping solution was added into each well.

About 10 minutes later, stop solution was added, and absorbance was measured using the ELISA reader at 450 nm with 630 nm as the reference wavelength.

Finally, a stabilized chromogen was added, and the colorimetric reaction was developed within 30 min, after which "stop solution" was added and the absorbance was measured on a spectrophotometer at 450 nm.

100 μL of stop solution was added to each well, and the absorbance was recorded at 570 nm using a 96-well plate reader.

The plates were washed 5 times with wash solution, chromogenic reagents A and B were added in turn for 10 min at 37 °C, and the stop solution was added.

Third, 100 μL of TMB one-step substrate reagent was added to each well to develop color; then, the stop solution was added to change the color from blue to yellow.

Finally, 50 μL of stop solution was added and the developed color was measured at 405 nm in a microtiter plate reader (Ravan and Yazdanparast 2012; Gomes et al. 2010; Di Pinto et al. 2012).

Then the stop solution was added to each well.

Stop solution was added after color development and the A450 was recorded.

Two microliters of stop solution was added and the sample mixed.