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Stopping solution: 2 N HCl. .
The reaction was stopped by 100 µl/well of stopping solution.
The reaction was stopped by addition of 20 μl stopping solution [100 nM ethylenediamine tetraacetic acid (EDTA), NaN3 (0.02 %), pH 8.0].
Substrate solution (100 μL) was added, and the reaction was kept for 15 min. Finally, stopping solution (2 N HCl) was used to stop the reaction, and the absorbance was determined at 492 nm.
The assay was developed with BluePhos substrate (Kirkegaard Perry, Gaithersburg, MD) for 15 min at room temperature, then stopped with stopping solution (Kirkegaard Perry) and read at 570 nm (SpectraMax Plus384, Molecular Devices).
After 15 minutes, the reaction is stopped with 100 μL of 0.18 M H2SO4 stopping solution.
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The reaction was terminated by stop solution.
Stop solution, 2 N H2SO4, was added to each well.
-Diluent 2: one vial contains lyophilized material of murine origin -Wash solution (20×): one 50 mL vial -Substrate: one vial -Stop solution: Stop solution is a tacrine solution.
The ELISA was stopped with 50 μl/well stop solution and read at OD 450 nm.
The reaction was stopped by adding 50 μL of 2 N H2SO4 "Stop" solution.
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