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Gametophyte cultures were placed on a shelf 9 cm below the stopping screen of the apparatus; 1100 psi rupture discs were used.
Then, the plates were placed under the stopping screen of a Biolistic PDS-1000/He PDS-1000/Helivery system (Bio-Rad) at a distance of 6 cm.
The microcarrier launch assembly was placed in the second slot from the top (level 2), and the target cells were placed at level 4 (the shortest distance from the stopping screen to the target cells).
The distance between macrocarrier and stopping screen (Bio-Rad) was set to 8 mm.
The distance between the macrocarrier and the stopping screen was set to 7 mm, and the distance between the stopping screen and target cells was adjusted to 8 cm.
The membrane filter with its layer of E. elegans cells was positioned in the bombardment chamber, and the distance between the target cells and the stopping screen was adjusted to 8 cm.
Similar(53)
The burst pressure of the rupture disks (Bio-Rad) was 1,100 psi, the rupture disk-macrocarrier distance was adjusted to 7 mm, the macrocarrier-stopping screen distance was 8 mm, the stopping screen-target cell distance was adjusted to 11 cm and the bombardment chamber was evacuated to 28 inch Hg.
The consequences of stopping screening prematurely could be disastrous.
The decision of stopping screening may also be supported by a negative result of HPV testing.
There is much controversy about what is the appropriate age of stopping screening.
We also found that physicians feel uncomfortable discussing stopping screening with women aged 80 and older.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com